Input Parameters


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CvP plot


The bias of Charged vs. polar residues (CvP-bias) has been shown to correlate with the thermostability for eukaryotic (prokayotic) proteins (c.f. References).

Note: If you compare orthologous sequences you might want to align the sequence first and only analyze the relevant regions

Number of charged amino acids (EDKR)
Number of polar amino acids (STNQ)
(Ncharged - Npolar)/length * 100
Larger CvP values imply higher thermostability


Holder et. al., 2013. Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability. Biology Direct 8:2 [Abstract]

Suhre & Claverie, 2003. Genomic correlates of hyperthermostability, an update. J Biol Chem 278:17198-17202 [Article]

Author / Contact

Steffen Schmidt
Max Planck Institute for Developmental Biology - Dept. Biochemistry
Tübingen, Germany