Input Parameters

 

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CvP plot

Description

The bias of Charged vs. polar residues (CvP-bias) has been shown to correlate with the thermostability for eukaryotic (prokayotic) proteins (c.f. References).

Note: If you compare orthologous sequences you might want to align the sequence first and only analyze the relevant regions

Ncharged
Number of charged amino acids (EDKR)
Npolar
Number of polar amino acids (STNQ)
CvP-bias
(Ncharged - Npolar)/length * 100
Note:
Larger CvP values imply higher thermostability

References:

Holder et. al., 2013. Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability. Biology Direct 8:2 [Abstract]

Suhre & Claverie, 2003. Genomic correlates of hyperthermostability, an update. J Biol Chem 278:17198-17202 [Article]

Author / Contact

Steffen Schmidt
Max Planck Institute for Developmental Biology - Dept. Biochemistry
Tübingen, Germany